This report describes the on-site commissioning of a peptide synthesizer at a pharmaceutical research institute and its validation through a complete solid-phase synthesis of thymopentin (TP-5). The peptide synthesizer was prepared through system software upgrade, reagent-line cleaning, flow-rate calibration, and method optimization, then verified with a full TP-5 run. Results confirmed stable operation and accurate reagent delivery suitable for routine peptide production.
PWe put a peptide synthesizer through a full TP-5 run on site
When a customer asks whether our peptide synthesizer can handle real production, the best answer is to run it. Our engineer went to a pharmaceutical research institute, set up the instrument, and synthesized thymopentin (TP-5) from scratch. A peptide synthesizer is only as good as the batch it actually delivers, so we treat every install like a live test. Here is what the run looked like and why it matters for buyers evaluating a peptide synthesizer.
What we checked before the first run
Upgraded the control software to the latest stable build on the peptide synthesizer.
Flushed every line with methanol and NMP, three passes each, so the peptide synthesizer starts clean.
Calibrated flow at each port so dosing stays on target during the whole run on the peptide synthesizer.
Running TP-5 on the synthesizer
Sequence Arg-Lys-Asp-Val-Tyr, built residue by residue on the peptide synthesizer.
| Site | Resin / amino acid (C to N) |
| 0 | Rink Amide AM Resin (C-terminal amide) |
| 1 | Fmoc-Tyr(tBu)-OH |
| 2 | Fmoc-Val-OH |
| 3 | Fmoc-Asp(OtBu)-OH |
| 4 | Fmoc-Lys(Boc)-OH |
| 5 | Fmoc-Arg(pbf)-OH |
90-minute couplings, 40°C bath, automatic washes between steps on the peptide synthesizer.
Kaiser tests after the first and last couplings confirmed the reaction was complete each time on the peptide synthesizer.
The numbers that matter
1.89 g crude from a 1.36 g theoretical target, yield above plan on the peptide synthesizer.
One overnight run, zero errors, zero leaks on the peptide synthesizer.
Stable temperature and clean reagent delivery throughout the run on the peptide synthesizer.
One problem we fixed
Resin was washing to the top of the reactor and throwing off the flow meter of the peptide synthesizer. A parameter tweak in the program solved it on the peptide synthesizer. Small fix, big impact on accuracy for any peptide synthesizer doing SPPS.
Why this matters when you buy
A peptide synthesizer that passes a live TP-5 run is a peptide synthesizer you can trust in production. The PepAxis line is built so that what we did on site is repeatable in your lab, and we size each peptide synthesizer to the sequence, not the other way around. If a peptide synthesizer cannot clear this bar, it should not be on your shortlist.
If you are evaluating a peptide synthesizer for your lab or plant, talk to us. The PepAxis line covers single-channel and multi-channel models built for exactly this kind of work. See the full range at pepaxis.com.
E-mail: echoliu@dilunbio.com